BMG Labtech

CLARIOstar Plus

Advanced Monochromator-based

Multi-mode Microplate Reader

Video

Overview

The most flexible and highly sensitive multi-mode microplate reader

The CLARIOstar^® Plus is BMG LABTECH's most flexible multi-mode microplate reader. Equipped with the patented dual LVF Monochromators™ system, highly sensitive filters, UV/Vis spectrometer and Enhanced Dynamic Range (EDR) technology, the CLARIOstar^® Plus combines flexibility with the best sensitivity of its class. The CLARIOstar^® Plus is a modular microplate reader and can be equipped with up to seven detection modes; UV/Vis Absorbance, Fluorescence intensity (incl. FRET), Luminescence (incl. BRET), Fluorescence polarization, Time-resolved fluorescence (TRF), Time-resolved FRET (TR-FRET) and Alpha Technology (AlphaScreen^®/AlphaLISA^®/AlphaPlex™).

Features & Benefits

Ultra fast spectral scanning in Absorbance (from 220 to 1000 nm in less than 1 second per well)
Well scanning with up to 900 data points per well for non-homogeneous samples (e.g. cell-based assays)
Increased sensitivity with LVF Monochromators™ over conventional monochromators
Ability to combine optical filters and LVF Monochromators™ in one measurement
Continuously adjustable bandwidths from 8 to 100 nm
Automated gain adjustment with Enhanced Dynamic Range (EDR) technology
Automated focus (focal height) adjustment for both top and bottom readings
High-precision injectors capable of simultaneous reagent injection and detection
Measurements in up to 1536-well microplate format
Linear, orbital and double-orbital shaking with user-definable time and speed
Incubation up to 65°C with 0.1°C resolution
Multi-user SMART Control Software with integrated fluorophore library and unlimited license
Multi-user MARS Data Analysis Software with unlimited license
FDA 21 CFR Part 11 compliance in software
Data export to Excel, Absolute Database or ASCII format
Easy integration with LIMS systems
Easy integration with robotic systems for heavy workload and lab automation
Compatibility with Atmospheric Control Unit (ACU) for O2 and CO2 gas regulation inside reader chamber
Compatibility with Evaluation Plate for GLP compliance
Compatibility with LVis Plate for low-volume (down to 2µl) samples

Technical Specifications

Detection Modes	UV/Vis Absorbance Fluorescence Intensity - incl. FRET Luminescence (flash and glow) - incl. BRET Fluorescence Polarization Time-resolved Fluorescence (TRF) Time-resolved FRET (TR-FRET) AlphaScreen^®/AlphaLISA^®/AlphaPlex™
Measurement Modes	Top and bottom (optional) reading Endpoint and kinetic measurements Sequential multi-excitation measurements Sequential multi-emission measurements Spectral scanning (ABS, FI, LUM) Ratiometric measurements Well scanning
Microplate Formats	6 to 384 or 1536 (optional) well plates, user-definable LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier	Robot compatible
Light Sources	High energy Xenon flash lamp Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex™
Detectors	Low-noise photomultiplier tube Red-sensitive photomultiplier tube (optional) CCD spectrometer (ABS)
Wavelength Selection	Dual Linear Variable Filter (LVF) Monochromators™ Linear Variable Dichroic Mirror Separates excitation and emission LVF Monochromators Optical Filters Excitation and emission slides hold up to 4 filters each LVF Monochromators + Optical Filters Use one for excitation and the other for emission UV/Vis Absorbance Spectrometer Full spectra or 8 discrete wavelengths in ‹1 sec/well
Optical Filters	Excitation and emission slides for up to 4 filters each
Optical Path Guides	Top and bottom: Free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment	Automatic focal height adjustment (0.1 mm resolution)
Spectral Range	Filters 240 - 740 nm or 240 - 900 nm (red-shifted PMT) for FI, FP, TRF 240 - 740 nm for LUM LVF Monochromators™ 320 - 740 nm or 320 - 840 nm (red-shifted PMT) for FI 320 - 740 nm for LUM Linear Variable Dichroic 340 - 740 nm or 340 - 760 nm (red-shifted PMT) Spectrometer 220 - 1000 nm for ABS Wavelength precision: ≤0.5 nm
Sensitivity	FI Filters (top) ‹0.15 pM (‹3 amol/well FITC, 384sv, 20 μL) FI Filters (bottom) ‹1.0 pM (‹50 amol/well FITC, 384g, 50 μL) FI Monochromator (top) ‹0.35 pM (‹7 amol/well FITC, 384sv, 20 μL) FI Monochromator (bottom) ‹3.0 pM (‹150 amol/well FITC, 384g, 50 μL) FI Dynamic Range 8 decades in a single measurement FP ‹0.5 mP SD at 1 nM FITC (384sv, 20 μL) HTRF^® (black and white microplates) Reader Control Kit (Eu) after 18h (384sv, 20 μL) Delta F ›880% (High Calibrator) Delta F ›30% (Low Calibrator) TRF ‹20 fM europium, 384, 80 μL LUM ‹0.4 pM (‹8 amol/well ATP, 384sv, 20 μL) LUM Dynamic Range 8 decades in a single measurement AlphaScreen^® with Laser ‹5 pM (‹100 amol/well P-Tyr-100, 384sv, 20 μL) ABS with Spectrometer Selectable spectral resolution: 1, 2, 5 and 10 nm OD range: 0 to 4 OD Photometric resolution: 0.001 OD Accuracy: ‹1% at 2 OD Precision: ‹0.5% at 1 OD and ‹0.8% at 2 OD Linearity: ≤0.8% at 2.0 OD
Read Times	Flying mode (1 flash): 8 sec (96), 15 sec (384), 28 sec (1536) 10 flashes: 19 sec (96), 57 sec (384), 3 min 4 sec (1536)
Shaking	Linear, orbital and double-orbital with user-definable time and speed
Incubation	+3°C above ambient up to 45°C or 65°C (optional) The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software	Multi-user SMART Control Software & MARS Data Analysis Software FDA 21 CFR Part 11 compliant Integrated fluorophore library
Dimensions & Weight	Width: 45 cm, Depth: 51 cm, Height: 40 cm; Weight: 32 kg
Optional Accessories	Atmospheric Control Unit (ACU)System to actively regulate O2 and CO2 (0.1-20%). Ability to perform gas ramping for hypoxic and ischemia/reperfusion assays, as well as for metabolic and redox experiments. Stacker IIIPlate handler for up to 50 microplates - continuous loading feature Reagent Dispenser ModuleUp to 2 built-in reagent injectors Individual injection volumes for each well: 3 to 500 μL (optionally up to 2 mL) Variable injection speed up to 420 μL/sec Reagent back flushing LVis PlateMicroplate designed to measure 16 low volume (2 μL) samples and standard cuvettes. Incorporates NIST-traceable filters and holmium oxide standards for instrument performance test. Sensitivity: ‹2 ng/μL dsDNA Evaluation PlatePlate designed to validate the optical functionality of microplate reader for quality control and/or qualification (IQ/OQ) purposes in environments following GLP compliance. THERMOstarMicroplate incubator and shaker Integrated Barcode ReaderUp to 2 integrated barcode readers FiltersOptical filters optimised for dyes, fluorophores and specific assays. Filters available for all applications from UV to NIR. Customised filters available upon request.

ABS: Absorbance / FI: Fluorescence Intensity / LUM: Luminescence / FP: Fluorescence Polarization / TRF: Time-resolved Fluorescence / TR-FRET: Time-resolved FRET FI (incl. FRET) is the standard detection mode. Other detection modes are optional.

All or some of the specifications and features noted on this page may be optional. All necessary details in this regard may not be specified on this page. Please contact us for full details. / Product design, appeareance, functionality, features and specifications are subject to change without notice.