BMG Labtech

FLUOstar Omega

Advanced Filter-based

Multi-mode Microplate Reader

Overview

The ideal multi-mode microplate reader for research

The FLUOstar^® Omega is a multimode microplate reader that can combine UV/Vis absorbance, Fluorescence intensity (incl. FRET and TRF) and Luminescence (incl. BRET) modes. It utilises an ultra-fast UV/Vis spectrometer for absorbance as well as highly sensitive filters for all other detection modes. The FLUOstar^® Omega is the ideal plate reader for life science applications.

Features & Benefits

Ultra fast spectral scanning in Absorbance (from 220 to 1000 nm in less than 1 second per well)
Well scanning with up to 900 data points per well for non-homogeneous samples (e.g. cell-based assays)
High-precision injectors capable of simultaneous reagent injection and detection
Measurements in up to 1536-well microplate format
Top and bottom reading in both fluorescence and luminescence applications
Linear, orbital and double-orbital shaking with user-definable time and speed
Incubation up to 65°C with 0.1°C resolution
Multi-user Reader Control Software with unlimited license
Multi-user MARS Data Analysis Software with unlimited license
FDA 21 CFR Part 11 compliance in software
Data export to Excel, Absolute Database or ASCII format
Easy integration with LIMS systems
Easy integration with robotic systems for heavy workload and lab automation
Compatibility with Stacker III for higher throughput
Compatibility with Atmospheric Control Unit (ACU) for O2 and CO2 gas regulation inside reader chamber
Compatibility with Evaluation Plate for GLP compliance
Compatibility with LVis Plate for low-volume (down to 2µl) samples

Technical Specifications

Detection Modes	UV/Vis Absorbance Fluorescence Intensity - incl. FRET and TRF Luminescence (flash and glow) - incl. BRET
Measurement Modes	Top and bottom reading Endpoint and kinetic measurements Sequential multi-excitation measurements Sequential multi-emission measurements Simultaneous dual emission measurements (optional; FI and LUM) Spectral scanning (ABS) Ratiometric measurements Well scanning
Microplate Formats	6 to 384 well plates for FI and LUM, 6 to 384 or 1536 (optional) for ABS; user-definable LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier	Robot compatible
Light Sources	High energy Xenon flash lamp
Detectors	Side window photomultiplier tube CCD spectrometer (ABS)
Wavelength Selection	Optical Filters Filter wheels for excitation and emission UV/Vis Absorbance Spectrometer Full spectra or 8 discrete wavelengths in ‹1 sec/well
Optical Filters	Excitation and emission filters wheels for up to 8 filters each
Spectral Range	Filters 240 - 740 nm or 240 - 900 nm (optional) for FI 240 - 740 nm for LUM Spectrometer 220 - 1000 nm for ABS Wavelength precision: ≤0.5 nm
Sensitivity	FI ‹0.2 fmol/well FITC TRF ‹30 amol/well europium LUM 20 amol/well ATP DLReady™ certified ABS with Spectrometer Selectable spectral resolution: 1, 2, 5 and 10 nm OD range: 0 to 4 OD Photometric resolution: 0.001 OD Accuracy: ‹1% at 2 OD Precision: ‹0.5% at 1 OD and ‹0.8% at 2 OD Linearity: ≤0.8% at 2.0 OD
Read Times	Flying mode (1 flash): 9 sec (96), 16 sec (384)
Shaking	Linear, orbital and double-orbital with user-definable time and speed
Incubation	+4°C above ambient up to 45°C or 65°C (optional) The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software	Multi-user Reader Control Software & MARS Data Analysis Software FDA 21 CFR Part 11 compliant
Dimensions & Weight	Width: 44 cm, Depth: 48 cm, Height: 30 cm; Weight: 28 kg
Optional Accessories	Atmospheric Control Unit (ACU)System to actively regulate O2 and CO2 (0.1-20%). Purge Gas VentSystem to inject an atmosphere or to pull a vacuum into the reader. Stacker IIIPlate handler for up to 50 microplates - continuous loading feature Reagent Dispenser ModuleUp to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well: 3 to 500 μL Variable injection speed up to 420 μL/sec Up to 4 injection events per well Reagent back flushing LVis PlateMicroplate designed to measure 16 low volume (2 μL) samples and standard cuvettes. Incorporates NIST-traceable filters and holmium oxide standards for instrument performance test. Sensitivity: 2 ng/μL dsDNA Evaluation PlatePlate designed to validate the optical functionality of microplate reader for quality control and/or qualification (IQ/OQ) purposes in environments following GLP compliance. THERMOstarMicroplate incubator and shaker FiltersOptical filters optimised for dyes, fluorophores and specific assays. Filters available for all applications from UV to NIR. Customised filters available upon request.

ABS: Absorbance / FI: Fluorescence Intensity / LUM: Luminescence / TRF: Time-resolved Fluorescence FI (incl. FRET and TRF) is the standard detection mode. Other detection modes are optional. FLUOstar Omega can be ordered with filter-based ABS instead of spectrometer-based ABS. LVis Plate requires spectrometer-based ABS to operate.

All or some of the specifications and features noted on this page may be optional. All necessary details in this regard may not be specified on this page. Please contact us for full details. / Product design, appeareance, functionality, features and specifications are subject to change without notice.