BMG Labtech

NEPHELOstar Plus

The World's First and Only

Microplate Nephelometer

Video

Overview

Laser-based microplate nephelometer for light-scattering and turbidity measurements

The NEPHELOstar^® Plus microplate nephelometer was developed to meet high-throughput demands for fast compound solubility screens. It detects insoluble particles in liquid samples by measuring forward scattered light and can be used for turbidity measurements, drug solubility screening, protein binding kinetics, microbial growth, and petroleum process control.

Features & Benefits

Adjustable laser intensity and beam diameter for best performance
High-precision injectors capable of simultaneous reagent injection and detection
Measurement in up to 384-well microplate format
Linear, orbital and double-orbital shaking with user-definable time and speed
Incubation up to 60°C with 0.1°C resolution
Multi-user Reader Control Software with unlimited license
Multi-user MARS Data Analysis Software with unlimited license
FDA 21 CFR Part 11 compliance in software
Data export to Excel, Absolute Database or ASCII format
Easy integration with LIMS systems
Easy integration with robotic systems for heavy workload and lab automation
Compatibility with Stacker III for higher throughput
Compatibility with Atmospheric Control Unit (ACU) for O2 and CO2 gas regulation inside reader chamber

Technical Specifications

Detection Modes	Nephelometry (light scattering)
Measurement Modes	Endpoint and kinetic measurements
Microplate Formats	Up to 384 well plates
Microplate Carrier	Robot compatible
Light Sources	Self-monitoring Laser Diode Wavelength: 635±10 nm Stability: ‹0.2% deviation Lifetime: 20,000 hours Output: 1 mW Selectable beam width: 1.5 to 3.5 mm Selectable intensity: 0-100% Scattering angle: Detects up to 80° full cone angle
Detectors	Side window photodiode detector
Wavelength Selection	Photodiode Wavelength: 635±10 nm
Sensitivity	Depends on particle size and liquid properties Silica detection (particle size 0.5 to 10 µm): 800 nM Dynamic range: 5 decades Maximum count rate: 2,000,000 Relative Nephelometry Units (RNUs) per second
Read Times	Depend on assay conditions and liquid surface stability Shortest times possible: 16 sec (96), 47 sec (384)
Shaking	Linear, orbital and double-orbital with user-definable time and speed
Incubation	+4°C above ambient up to 45°C or 60°C (optional) The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software	Multi-user Reader Control Software & MARS Data Analysis Software FDA 21 CFR Part 11 compliant
Dimensions & Weight	Width: 44 cm, Depth: 48 cm, Height: 32 cm; Weight: 25 kg
Optional Accessories	Atmospheric Control Unit (ACU)System to actively regulate O2 and CO2 (0.1-20%). Purge Gas VentSystem to inject an atmosphere or to pull a vacuum into the reader. Stacker IIIPlate handler for up to 50 microplates - continuous loading feature Reagent Dispenser ModuleUp to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well: 3 to 500 μL Variable injection speed up to 420 μL/sec Up to 4 injection events per well Reagent back flushing THERMOstarMicroplate incubator and shaker

All or some of the specifications and features noted on this page may be optional. All necessary details in this regard may not be specified on this page. Please contact us for full details. / Product design, appeareance, functionality, features and specifications are subject to change without notice.